{"product_id":"simple-dna-extraction-methods-from-human-blood-9783659536342","title":"Simple DNA Extraction Methods from Human Blood","description":"This study was aimed to compare between four DNA extraction methods, including phenol-chloroform-isoamyle, guanidine hydrochloride, saturated sodium chloride, and chelex-100 using 50 blood samples collected from healthy volunteers. The comparison included DNA concentration, extracted DNA purity, cost of reagents per one sample, and time consuming. After a comprehensive analysis of all factors: salt extraction gave the maximum yield of DNA, it is saver and simpler than the other methods. Moreover, this method is reliable and inexpensive. But its purity is slightly low. Phenol-chloroform-isoamyl extraction method give reliable quantity and purity of the DNA extracted. But it takes a time and labor in obtaining pure extract. Moreover, its associated toxicities warrant a judicious use. Chelex-100 resin extractions gave a DNA quantity as well as phenol-chloroform- isoamyle extraction. It is save, simple, does not require any organic solvent, and takes very short time and a little possibility of cross contamination. But it is expensive. Guanidine hydrochloride extraction is an expensive method taking a long time with a low quantity and quality of the DNA obtained.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAuthor:\u003c\/b\u003e \u003ca href=\"https:\/\/sureshotbooks-com.myshopify.com\/search?type=product%2Carticle%2Cpage\u0026amp;q=AUTH-8633431\"\u003eAli Samar\u003c\/a\u003e\u003cbr\u003e\u003cb\u003ePublisher:\u003c\/b\u003e LAP Lambert Academic Publishing\u003cbr\u003e\u003cb\u003ePublished:\u003c\/b\u003e 04\/24\/2014\u003cbr\u003e\u003cb\u003ePages:\u003c\/b\u003e 56\u003cbr\u003e\u003cb\u003eBinding Type:\u003c\/b\u003e Paperback\u003cbr\u003e\u003cb\u003eWeight:\u003c\/b\u003e 0.21lbs\u003cbr\u003e\u003cb\u003eSize:\u003c\/b\u003e 9.00h x 6.00w x 0.13d\u003cbr\u003e\u003cb\u003eISBN13:\u003c\/b\u003e 9783659536342\u003cbr\u003e\u003cb\u003eISBN10:\u003c\/b\u003e 3659536342\u003cbr\u003e\u003cb\u003eBISAC Categories:\u003c\/b\u003e\u003cbr\u003e- \u003ca href=\"https:\/\/sureshotbooks-com.myshopify.com\/search?type=product%2Carticle%2Cpage\u0026amp;q=CAT-MED\"\u003eMedical\u003c\/a\u003e | \u003ca href=\"https:\/\/sureshotbooks-com.myshopify.com\/search?type=product%2Carticle%2Cpage\u0026amp;q=BISAC-MED000000\"\u003eGeneral\u003c\/a\u003e\u003cbr\u003e\u003cp\u003e\u003ci\u003eThis title is not returnable\u003c\/i\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"LAP Lambert Academic Publishing","offers":[{"title":"Default Title","offer_id":44697270452461,"sku":"9783659536342","price":51.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0550\/8097\/6621\/products\/img_ba828030-99e7-42d1-b49c-3452d4f077a0.jpg?v=1703815040","url":"https:\/\/sureshotbooks.com\/products\/simple-dna-extraction-methods-from-human-blood-9783659536342","provider":"SureShot Books Publishing LLC","version":"1.0","type":"link"}